Friday, December 27, 2019

The Use Of Recombinant Dna For Science And Medicine

Introduction The use of recombinant DNA technology has been a huge advancement to science. In the early 1970s the first recombinant DNA was produced (â€Å"1972 First†, 2013). Researchers Stanley Cohen and Hebert Boyer used restriction enzymes to cut DNA at specific sites, and then fused them back together to form their recombinant vector (â€Å"1972 First†, 2013; Cederbaum, S., et al., 1984). Through transformation the vector is then inserted into a host cell to amplify their hybrid DNA, also known as recombinant DNA (â€Å"1972 First†, 2013). This technology allowed scientists to insert a human gene into the genetic material of common bacteria. This was a huge break through for science and medicine. Now the recombinant DNA could be used to produce protein encoded by the human gene (â€Å"1972 First†, 2013; Cederbaum, S., et al., 1984). The first protein to be synthesized from recombinant DNA technology was human insulin (Tuohy, P., 2013). To do this the human insulin gene was inserted into a plasmid, which had previously been cut by restriction enzymes. After the insulin sequence was added into the plasmid, the plasmid was then transformed into bacteria to amplify the insulin sequence (Tuohy, P., 2013). The new recombinant bacteria are then induced, and the bacteria produce more of the insulin. The insulin is then harvested and purified, so that it can be used to treat diabetes (Tuohy, P., 2013). Before insulin was produced through genetic engineering, it had to be obtained fromShow MoreRelatedThe Human Of Recombinant Bacteria1649 Words   |  7 PagesRecombinant bacteria is a bacterium cell that has had its genome changed, by the alteration, or edition of a gene. 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